| Intrument | Microplate reader(495-525 nm,optimum wavelength: 510 nm) |
| Assay Time | 20 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum, plasma, tissue |
| Synonyms | FC |
| Detection Principal | Free cholesterol produces 4-cholestenone and hydrogen peroxide under the oxidation of cholesterol oxidase. In the presence of 4-aminoamylpyridine and phenol, peroxidase catalyze hydrogen peroxide to form red quinone compounds of benzoquinone imine phenizone. The color depth of the generated quinones is directly proportional to the cholesterol content. |
| Reagents | Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4), Isopropanol(AR) |
| Labware | Micropipettor, Incubator, Vortex mixer, Centrifuge |
| Size | 96T |
| Sensitivity | 0.07 mmol/L |
| Detection Range | 0.07-24 mmol/L |
| Inter CV | 5.2 |