| Intrument | Microplate reader(535 nm-540 nm,optimum wavelength: 540 nm) |
| Assay Time | 40 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Plant tissue |
| Detection Principal | The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance. ?-amylase was inactivated by the property of amylase not to be heat-resistant, and then the enzyme activity of total amylase and ?-amylase is determined. So the activity of ?-amylase can be calculated indirectly. |
| Labware | Test tubes, Vortex Mixer, Centrifuge, Water bath |
| Size | 96T |
| Sensitivity | 0.97 U/g tissue |
| Detection Range | 0.97-34.74 U/g tissu |
| Recovery Rate | 96 |
| Inter CV | 3.2 |