| Intrument | Microplate reader(535 nm-540 nm,optimum wavelength: 540 nm) |
| Assay Time | 30 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | Serum, plasma, saliva, tissue |
| Detection Principal | The reducing sugar reacts with 3,5-dinitrosalicylic acid under heating conditions to produce a brown-red substance. Amylase activity can be calculated by measuring the OD value at 540 nm. |
| Labware | Test tubes, Vortex Mixer, Centrifuge, Water bath |
| Size | 96T |
| Sensitivity | 0.008 U/mL |
| Detection Range | 0.01-0.56 U/mL |
| Recovery Rate | 97 |
| Inter CV | 3.6 |