| Intrument | Spectrophotometer(520 nm) |
| Assay Time | 45 min |
| Validity | 6 |
| Assay Type | Quantitative |
| Sample Type | Serum, plasma, animal tissue |
| Detection Principal | Under the action of acidic solution and reductant, ferric ions can be separated from transferrin in serum, and reduced into ferrous ions (Fe2+). The latter then bind to bipyridine and form pink complexes. The concentration of iron can be calculated by measuring the OD value at 520 nm indirectly. |
| Labware | Test tubes, Vortex Mixer, Centrifuge, Water bath, Test tubes, Vortex Mixer, Centrifuge, Water bath |
| Size | 100 Assays |
| Sensitivity | 0.08 mg/L |
| Detection Range | 0.08-60 mg/L |
| Recovery Rate | 97 |
| Inter CV | 3.2 |