| Intrument | Microplate reader (500-520 nm, optimum wavelength: 505 nm) |
| Assay Time | 70 min |
| Validity | 6 |
| Assay Type | Enzyme Activity |
| Sample Type | animal tissu |
| Detection Principal | Sucrase catalyzes its substrate (sucrose) to produce glucose, which produces hydrogen peroxide under the action of glucose oxidase. Hydrogen peroxide reacts with chromogenic agent to produce red substance, which has a strong absorption peak at 505 nm. In a certain concentration range, It's absorbance is proportional to glucose concentration. Therefore, the activity of sucrase can be calculated by measuring the OD value at 505 nm. |
| Reagents | PBS (0.01 M, pH 7.4) |
| Labware | Micropipette, Vortex mixer, Centrifuge |
| Size | 96T |
| Sensitivity | 20 U/mL |
| Detection Range | 20-2000 U/mL |
| Recovery Rate | 100 |
| Inter CV | 6.5 |